Formulasi Spray Kombucha Bunga Telang (Clitoria ternatea L.) Berbasis Kearifan Lokal Rangkasbitung sebagai Produk Bioteknologi Pertanian dan Farmasi Antifungi terhadap Fusarium oxysporum pada Komoditas Hortikultura

Abstrak

The utilization of butterfly pea flower (Clitoria ternatea L) as a local resource represents an important form of local wisdom that can be developed into innovative pharmaceutical biotechnology products. Butterfly pea flower contains various bioactive compounds with potential antimicrobial and antifungal properties. Through kombucha fermentation, these bioactive components may be transformed or enhanced, providing opportunities for the development of natural antifungal preparations. This study aimed to formulate and evaluate a kombucha-based spray preparation using butterfly pea flower as a locally sourced active ingredient from Rangkasbitung, Banten, and to determine its antifungal activity against Fusarium oxysporum, a pathogenic fungus associated with diseases and infections in horticultural crops. The spray formulations were prepared using different concentrations of butterfly pea flower kombucha, namely 20%, 30%, and 40%. The antifungal activity was determined using the inhibition-zone method by measuring the diameter of fungal growth inhibition produced by each treatment. The data were statistically analyzed using one-way analysis of variance (ANOVA), followed by post hoc analysis to identify significant differences among treatments. The results demonstrated that the ANOVA analysis produced p-values below 0.05, indicating significant differences in antifungal activity among the tested concentrations. The post hoc analysis also showed significant differences, particularly between the 20% and 40% treatments. Among the formulations, the 40% kombucha spray demonstrated the highest antifungal activity against F. oxysporum, with an average inhibition-zone diameter of 15.05 mm, categorized as a strong antifungal response. These findings indicate that butterfly pea flower-based kombucha, rooted in local wisdom of Rangkasbitung, Banten, has promising potential to be developed as a natural pharmaceutical biotechnology spray for controlling F. oxysporum in horticultural crops.